Antioxidant properties from seaweeds Kappaphycus alvarezii, Euchema spinosum and Sargasum sp. using different solvent

Abstract

Abstract Antioxidants are substances produced by the body in small amounts. Antioxidants could be obtained from seaweeds’ bioactive compounds. Seaweeds’ bioactive materials include sulfate polysaccharides, proteins, pigments caretonoid, polyunsaturated fatty acids (PUFA), essential minerals, vitamins and other secondary metabolites. The aim of this research is to determine the antioxidant properties using DPPH radical scavenging method from the extracts of K. alvarezii, E. spinosum and Sargassum sp.. The experiment was conducted using three species of seaweeds (K. alvarezii, E. spinosum and Sargassum sp.) which were extracted with three different polarity solvents (n-hexane, ethyl asetate and ethanol). The results showed the presence of the antioxidant activity in various extraction of seaweeds. Inhibitor to DPPH Free Radical activity were shown in 100 ppm by extracts Sargassum sp. (etil asetat) 23.8±0.7 %, Sargassum sp. (ethanol) 11.7±0.4%, K. alvarezii (etil asetat) 14.7±0.4%, K. alvarezii (ethanol) 14.9±0.4%, E. spinosum (etil acetate) 12.1±0.4%, E. spinosum (ethanol) 12.0±0.4%, and BHT 58.5± 3.1%. All of the n-hexane extracts of the seaweeds had no antioxidant activities when being treated by qualitative of DPPH methods.