Examination of Taura Syndrome Virus (TSV) in white shrimp (Litopenaeus vannamei) and tiger prawn (Penaeus monodon) with Polymerase Chain Reaction (PCR) method

Abstract

Abstract Frozen shrimp is one type of fishery product. Fishery products are a type of food that is susceptible to damage due to pathogenic microbial contamination. Virus is a type of pathogen that can attack fishery commodities including shrimp. One type of virus that attacts shrimp namely Taura Syndrome Virus (TSV). This study aims to detect Taura Syndrome Virus in frozen white shrimp (Litopenaeus vannamei) and tiger prawn (Penaeus monodon) products. The research was carried out in December 2019 - January 2020 at the Fish Quarantine Center, Quality Control and Safety of Fishery Products in Surabaya II. Materials used in this study consist of two types of frozen shrimp, namely 5 samples of tiger prawn and 2 samples of white shrimp. Virus testing samples were detected molecularly using the conventional Polymerase Chain Reaction (PCR) method which consisted of an extraction stage using a Silica Extraction Kit, an amplification stage, electrophoresis and result visualization. This research was observative, then the data obtained were analyzed descriptively. The results showed that all shrimp samples consisting of white shrimp dan tiger prawn were negative of the Taura Syndrome Virus (TSV).