Antioxidant activity of endophytic fungi from young and old leaves of cinnamon plants from Bogor, Indonesia

Abstract

Abstract In addition to the bark, the leaves of the cinnamon plant are a source of natural antioxidant compounds and inhabited by endophytic fungi. The use of endophytic fungi from cinnamon leaves as a source of antioxidant compounds is not much done. This study aimed to determine the antioxidant activity of endophytic fungi were isolated from young and old leaves of cinnamon plants. Isolation of endophytic fungi was done by planting leaf tissue on Potato Dextrose Agar media after surface sterilization with 70% ethanol for 1 minute, 5.3% sodium hypochlorite for 5 minutes, and 70% ethanol for 30 seconds, respectively. Antioxidant activity is carried out based on the α, α-diphenyl-β-picrylhydrazyl free radical scavenging method. The ethyl acetate extract of endophytic fungi with the highest antioxidant activity on each type of leaf was carried out by profiling chemical compounds using GCMS. The extracts of eight endophytic fungal isolates obtained had antioxidant activity. Cb.Dm3 and Cb.Dt2 endophytic fungal extracts had the highest antioxidant activity with IC50 values of 62.80 and 8.11 µg.ml−1, respectively. Profiling chemical compounds showed that both isolates contained antioxidant compounds with Cb.Dt2 isolate containing cinnamaldehyde. Cb.Dt2 endophytic fungal extract has the potential as a potential source of natural antioxidant compounds.