In vitro anthelmintic activity of kersen leaf (Muntingia calabura) infusion against to Haemonchus contortus worm

Abstract

Abstract The problem caused by the gastrointestinal parasite has caused economic losses in the centres of ruminant livestock throughout the world. Parasitic resistance to synthetic antiparasitic, led researchers to explore alternative herbs as bio-anthelmintic. This study aims to determine the in vitro effect of M. calabura leaf infusion on egg hatchability (EHI) and mortality of adult female H. contortus worms (AWM), as a parameter of the antiparasitic properties of the leaves of the plant. The leaf infusion of M. calabura at doses of 2%, 4% and 6% was used for both treatments and albendazole at a dose of 2 mg/mL was used as a positive control. The negative control was sodium chloride at 0.9% (w/v). The EHI assay was conducted two times, before and 24 h after treatment, while AWM was monitored 15 and 30 min, and 1, 2, 3, 4, 5, 6, 7, and 8 h post test. The result showed that M. calabura leaves contain secondary metabolites, one of which was condensed tannins detected in this study. The leaf infusion of M. calabura at a concentration of 6% significantly inhibited EHI and AWM, higher than the negative control (P<0,05). While, it was not significantly different from albendazole 2 mg/ml on EHI test. The results conclude that the leaf infusion of M. calabura is fully potential as a bioanthelmintic against H. contortus worm.