Expression ChiPut-II gene from Serratia plymuthica UBCR_12

Abstract

Abstract Putative chitinase II gene [ChiPut-II] was isolated from rhizosphere bacteria strain UBCR_12 [NCBI accession number KX863673]. The ChiPut-II gene encodes chitinase enzyme which has the ability as a bio fungicide. Expression of the gene could be identified in the extracellular as well as in the intracellular of the host cell. The chitinolytic ability of the ChiPut-II enzyme effectively works on 3% colloidal chitin exhibiting specific activity up to 1.235 U/μg. The inhibition capacity of the ChiPut-II enzyme extracted from intracellular against Colletotrichum gloeosporioides is 15.59%. It’s chitinolytic and inhibition activity indicated the bacteria to be developed as a bio fungicide.