The Effect of Metformin on some Physiological Traits in Rats with Alloxan-Induced Diabetes

Abstract

Abstract The study was conducted in the laboratories of the University of Mosul, College of Veterinary Medicine, and the biological experiment was conducted in the animal house belonging to the College of Veterinary Medicine for the period from 3/1/2023 to 10/2/2023. The study included the use of the drug metformin to determine its effect on male rats with Alloxan-induced diabetes. The study included We used (24) adult male white rats, whose ages ranged from (13 to 14 weeks), and whose weights were recorded between (270 to 280 grams), at a rate of eight rats for each group, which included three groups, which were divided as follows: A healthy control group (T1), one with Alloxan-induced diabetes (T2) that received no treatment whatsoever, and a third with metformin, a chemotherapeutic drug, administered orally at a dosage of 200 mg/kg of body weight, were the three groups. The percentages of glucose, creatine, urea, glutathione (GSH), and malondialdehyde (MDA) were measured in blood tests. The levels of glucose, creatine, urea, and malondialdehyde (MDA) decreased significantly (P<0.05) in the metformin-dosed treatment group as compared to the control group. The results also shown that the percentage of glutathione (GSH) increased significantly in the same treatment (T3) compared to the control group with Alloxan-induced diabetes, at the level (P<0.05).