Influence of inbreeding on the morphobiological characteristics of meadow clover (Trifolium pratense L.)

Abstract

Abstract The influence of inbreeding on the morphobiological characteristics of meadow clover was revealed. Self-pollination, repeated in the number of successive generations, leads to an increase in homozygosity and to inbred depression, which increases from I1 to I3 generation and stabilizes in the I4 generation. It was found that in the I1 generation, according to the main morphobiological characteristics, there is no inward depression, but the maximum release of recessive lethal mutations is manifested, which amounted to 6.2%, and the survival rate of seedlings decreases (91.7%). By the I4 generation, the number of chlorophyll-free seedlings decreases to 1.4%. All the main morpho-biological indicators that determine the productivity of plants decrease from generation I1 to generation I3 by 1.5-2 times and stabilize in generation I4. Obtaining hybrid F1 offspring by crossing a linear material with an I4 induction level leads to the restoration of plant productivity indicators. When creating a linear material, an increase in the number of highly self-compatible genotypes from I1 to I4 generation by 60% is clearly traced. The data obtained make it possible to take into account the survival rate of seedlings, the cleavage of lethal and semi-lethal mutations, the degree of inbred depression in the formation of sample volumes when creating a linear material of meadow clover.