In vitro propagation of red ginger (Zingiber officinale Roxb. var. Rubrum) in different concentrations of sucrose and growth regulator

Abstract

Abstract In vitro culture of ginger is highly influenced by medium formulations such as sucrose and growth regulator. A micro-propagation was done to determine the influence of sucrose concentration and growth regulator on in vitro growth of red ginger. Sterilized sprouts with shoot tips collected from the rhizome were cultured on formulated media of MS medium containing three diverse concentrations of sucrose and different types and combinations of growth regulators for shoot differentiation. The number of days required for buds to break varies from 13 - 36 according to the sucrose concentration, type, and combinations of growth regulators. Among the formulated medium, the MS medium contained 30 gr of sucrose and BA 1 mg.l-1 induced bud sprouts in about 13-14 days. The sucrose concentration and growth regulator also had significant differences in shoot number, leaf number, and root number. Interaction of BA 1 mg.l-1 and sucrose 30 g.l-1 produced the highest number of shoots per explant (6.33) and the number of leaves per shoot (3.72). Auxin promotes the growth of intact roots as the medium supplemented with NAA yielded a higher number of roots, however, rooting was also observed in all formulated media after two passages of subculturing.