Identification of growth hormone gene polymorphism and its association with body weight in PO Kebumen cattle

Abstract

Abstract PO-Kebumen cattle was one of local genetic resources that famous with good body performance and high growth rate. The aim of this research was to detect growth hormone (GHR, GH and Pit-1) polymorphism and its association with body weight performance of PO-Kebumen cattle. The sample used in this research was 69 heads PO-Kebumen cattle belongs to the farmers of PO-Kebumen Cattle Farmer Association (ASPOKEB). This research observed the growth of PO Kebumen cattle, birth weight, weaning weight and the weight at one year. The parameters observed were birth weight, weaning weight and the weight at one year. The DNA extraction was carried out using blood samples. After DNA extraction, PCR-RFLP method was used to observe the genotype of three growth gene (GH, GHR and Pit1). The results showed that the GH and GHR genes were polymorphic, meanwhile Pit1 gene was monomorphic. The analysis showed that the three growth genes were not significantly associated to body weight (birth weight, weaning weight and the weight at one year) on PO-Kebumen cattle.