The effect of pineapple [Ananas comosus (L.) Merr.] juice in extender on Garut ram (Ovis aries L.) spermatozoa quality postcryopreservation

Abstract

Abstract This study was intended to determine the effect of various concentrations of pineapple [Ananas comosus (L.) Merr.] juice in extender on garut ram (Ovis aries L.) spermatozoa quality 24 h postcryopreservation. Semen samples were collected from five garut rams once a week using an artificial vagina. The samples were diluted in Tris-egg yolk extender with pineapple juice at concentrations of 0 % (KK), 5 % (KP 1), 10 % (KP 2), 15 % (KP 3), and 20 % (KP 4). The diluted semen samples were loaded into mini straws (0.25 mL) with 50 million cells/ml dosage. Samples were equilibrated at 5 °C for 2 h, and then frozen and stored in liquid nitrogen. Parameters evaluated were motility, viability, membrane integrity, acrosome integrity, and morphological abnormality. One-way ANOVA followed by Duncan’s test showed significant differences (P < 0.05) between KK and KP 3 in the percentage of motile spermatozoa and membrane integrity. Pineapple juice at 15 % could minimize the reduction of spermatozoa quality based on the percentage of motile spermatozoa (52.19 ± 5.32 %) and membrane integrity (38.52 ± 4.85 %).