Genetic stability of in vitro propagated transgenic Acacia mangium expressing xyloglucanase

Abstract

Abstract In 2011, a gene transformation experiment in A. mangium was carried out to yield transgenic A. mangium that overexpresses the xyloglucanase gene, enabling plants to grow faster. The transgenic A. mangium has been maintained and continuously subcultured since. Here, we tested the stability of the xyloglucanase gene in transgenic A. mangium following in vitro propagation. Molecular and morphological approaches were used. For all specimens tested, the wild-type A. mangium (K) and two lines of transgenic A. mangium named X11 and X21 were positive for the xyloglucanase gene after recurring subculture. Morphological observations did not reveal any alterations, and the cultures had undergone notable physiological deterioration.