Degeneration of kidney cells in high cholesterol-feeding rats treated with a combination of dadih and black glutinous rice: histology perspective

Abstract

Abstract Hypercholesterolemia can be harmful to cells, including the kidney cells. Fermented buffalo milk, “dadih” and black glutinous rice flakes “ampiang” are believed to be able to prevent hypercholesterolemia. This study aimed to analyze and identify histological changes in the kidneys of high cholesterol-fed rats treated with ampiang and dadih. Rats were distributed into three treatment groups: (A) negative control group given distilled water, (B) hypercholesterolemia group given 1% cholesterol, and (C) hypercholesterolemia prevention group given 1% cholesterol, ampiang, and dadih. All rats were treated for 35 days. Kidneys were collected by necropsy procedure on day 36. Kidney tissue was then prepared for histology using hematoxylin-eosin staining. The results showed hydropic degeneration of renal tubules in all treatment groups, with the highest percentage in the hypercholesterolemia group (B) (P<0.05). The percentage of degeneration in the group treated with ampiang and dadih (C) was the same as that in the negative control (A) (P>0.05). In the hypercholesterolemia group (B), necrosis, hemorrhage, and congestion were observed, while the other groups did not. Giving black glutinous rice flake ampiang and fermented buffalo milk dadih to prevent hypercholesterolemia can significantly prevent damage to the kidney organs.