Phenotypic and Genotypic Identification to S. aureus isolates from Cattle in Bogor

Abstract

Abstract S. aureus, commonly referred to as S. aureus, is a bacterium that responsible for a variety of infectious diseases that affect human being and animal health. Target of this study is to detect and clarify S. aureus phenotypically and genotypically using isolates originally derived from cattle. Phenotypic identification involves assessing biochemical characteristics such as Gram staining, catalase, and coagulase tests. Genotypic identification relies on detecting the presence of specific genes 23SrRNA and nuc associated with S. aureus through polymerase chain reaction (PCR) using appropriate primers. In this investigation, the focus of the genotypic detection PCR is on targeting the nuc genes, which serve as virulence factors. Out of the isolates tested, seven were identified as positive for the nuc genes, indicating the existence of the virulence factor in the S. aureus strain under study.