Abstract
Abstract
The use of agricultural production facilities that are produced from non-renewable natural resources such as fertilizers and chemical pesticides is constantly feared that will disrupt environmental sustainability and reduce soil fertility and quality. The application of biotechnology derived from local resources is a very appropriate alternative to answer these challenges, such as the use of local microorganisms that act as biological fertilizers and pesticides. The research aims to determine the compatibility of the bacterium Bacillus cereus strain ATCC 14579, Bacillus subtillis subsp. Subtilis strain 168 Bacillus siamensis strain KCTC13613, Azotobacter sp. and Pseudomonas fluorescens isolated from local microorganisms (MOL) banana weevil and know the best formulation, as well as the shelf life of the formula. The study was conducted in 3 stages: 1) testing bacterial compatibility on TSA media with the dual culture method; 2) test bacterial formulations on compost carriers, peat soils and vermicompost.3) Test the shelf life of formulas. The results showed that B. cereus strain ATCC 14579, B. subtillis subsp.subtilis strain 168, B. siamensis strain KCTC13613, P. fluorescens and Azotobacter sp were synergistic (compatible) so that they could be sponsored and formulated on carrier material (compost, kascing, peat). The best formulation is a formula for compost and peat soils added with Molas, CMC and Arginine. This formula has a shelf life of up to 6 months with the number of bacterial consortium colony of 4.64-4.67x 10 5 CFU / gram, fulfilling the criteria of biological fertilizer according to Ministry of Agriculture Decree No. 261 KPTS SR 310 4, 2019 which is ⩾ 105.