The Role of Sodium Chloride, Putrescine, and Melatonin on The Chemical Markers of Green Beans

Abstract

Abstract The purpose of this study was to evaluate the effects of salt stress using four different concentrations of sodium chloride (0, 40, 20, 60) mmol. L-1 and four different concentrations of the growth regulators putrescine and melatonin (0, PUT 0.2, MEL 0.2, MEL 0.4) mmol. L-1 and their overlap in some chemical markers of a green bean plant. The experiment was carried out using the complete random design (CRD) as a factorial experiment with two factors and ten repetitions. The results showed that the effect of salt stress led to a significant decrease in most of the chemical traits as it resulted in a concentration of 60 mmol. L-1 led to a decrease in the percentage of nutrients, Nitrogen Potassium, and a significant increase in sodium percentage in shoots compared to the control treatment (no growth regulator was added). While the percentage of phosphorous in the shoots was decreased by a concentration of 40 mmol. L-1 compared to a concentration of 20 mmol. L-1 which gave the highest percentage. The results showed that the addition of melatonin led to a significant increase in most of the studied traits when using concentrations 0.2 and 0.4 mmol. L-1, in the percentage of nutrients nitrogen, phosphorous, and potassium content in shoots, compared with the cultures grown under the influence of putrescin at a concentration of 0.2 mmol. L-1, which gave the lowest rate. As it gave the interaction treatment without adding sodium chloride with melatonin at a concentration of 0.4 mmol. L-1 had the highest percentage of nitrogen in the shoots. The treatment gave 20 mmol. L-1 NaCl with melatonin at a concentration of 0.4 mmol. L-1 had the highest percentage of phosphorus in the shoots. While the concentration was recorded at 60 mmol. L-1 NaCl with 0.4 mmol Melatonin. L-1 has the highest level of sodium content.