Comparative Study Between the Growth Regulators Benzyl Adenine (BAP) and Thydizoronate (TDZ) and Nano Iron in the Growth and Multiplication of Citrumelo Shoots in Vitro

Abstract

Abstract Research was conducted at Al-Qasim Green University in the Plant Tissue Culture Laboratory in the Department of Horticulture and Landscaping in the College of Agriculture from November 2021 to May 2022 with the aim of comparing two types of cytokinins: BAP at a concentration of 2.0 mg/L-1 and TDZ at a concentration of 0.2 mg/L-1 and two concentrations Iron (2.5-5 mg/L-1). To know their effect on the average of shoot multiplication in vitro of the root and its content of protein and macro (N.K) and Micro (iron) elements. The research was implemented as a factorial experiment using a complete random design (CRD) with two factors: cytokinin and nano iron. The results indicated a superiority of 0.2 mg.L-1 TDZ in each of the Multiplication shoots (4.667 shoots/explant ) and the content of shoots of iron (54.66 mg.kg-1), while BAP at a concentration of 2.0 mg/L-1 was excelled on the content of shoots of nitrogen, potassium and protein (1.778%, 1.947%.) respectively. As for nano iron at a concentration of 2.5 mg. L-1 was significantly excelled in the average of multiplication shoots (5 shoots/explant ), nitrogen (1.765%) and protein (10.98%). Specifically, the 5 mg dose. Potassium and iron (1.692% and 55.0 mg.kg-1) were most abundant in L-1.