Comparative study of antioxidant effect from extract and fraction of ‘Paku Atai Merah’ (Angiopteris ferox Coupel)

Abstract

AbstractPaku Atai Merah tubers with the Latin nameAngiopteris feroxCoupel have been used by the Dayak people as empirical treatment and have anticancer and antioxidant activity. The purpose of this study was to find out more the movement of extracts and fractions of Paku Atai Merah tubers in reducing free radicals using the total antioxidant capacity (TAC), CUPRAC, and Iron chelating methods. In this study, the sample used was the ethanol extract (EE) ofA.feroxCopel and then partitioned using n-hexane (FHP), ethyl acetate (FEAP), and aqueous-ethanol (FEP) solvents. The effectiveness of the sample in reducing free radicals with various antioxidant assay methods. The results obtained from this study indicate that the extract and fraction of Paku Atai Merah tubers have good effectiveness as an antioxidant agent. The total antioxidant capacity (TAC) method showed that EAP had a total antioxidant capacity of 101.60 μM/mg QEAC compared to EE (44.62 μM/mg QEAC), FEAP (89.88 μM/mg QEAC), and FHP (81.21 μM/mg QEAC). In the CUPRAC method using gallic acid as a standard for comparison, the results obtained were EE (4.00 μM/mg GEAC), FHP (1.45 μM/mg GEAC), FEAP (8.66 μM/mg GEAC), and APA (8.72 μM/mg GEAC). The antioxidant activity using the chelating iron method in each sample of EE, HPA, EAP, and APA showed activity with IC50 values of 384 μg/mL, 387.41 μg/mL, 339.23 μg/mL, and 347.3 μg/mL. Based on the results obtained from this study, it can conclude that the extract and fraction of Paku Atai Merah tubers have an excellent antioxidant agent to develop as a food supplement.