Exploration of indigenous rhizobacteria: in search for their potential as plant growth promoting bacteria at two potato producing areas in West Sumatra

Abstract

Abstract An experiment aiming at exploring and identifying indigenous rhizobacteria from Nagari Alahan Panjang and Nagari Batagak, two potato growing field at the Province of West Sumatra, has been conducted from July to October 2018. Soil was collected from the potato growing areas at Municipality Solok and Municipality Agam. One hundred grams of soil was collected from 25 spots of land at each area. Ten gram of soil was added to 9 mL of sterile distilled water prior to thorough mix in a vortex. The suspension was then subject to series of dilution to get 10-5 or 10-6 solution. Then, 0.1 mL of the final solution was poured into a test tube containing liquid NA medium and mixed thoroughly. The mixture was incubated for 48 hours at ambient temperature. Bacterial colonies went through series of re-culture until pure isolate was obtained and were observed for their morphological and physiological characters. Based on hypersensitivity reaction on leaves of Mirabilis jalapa, seventeen isolates found at Municipality Solok and 49 isolates were identified from Municipality Agam. These isolates did not cause leaf-tissue damage of Mirabilis jalapa. Different types and characters of the rhizobacteria is a broad range of biodiversity which will be potential for further screening for bio assays againts major weeds in potato cultivation and may be used to open the windows to the natural immunity and wellness through induced resistant to major weeds.