Identification and Phylogenetic Analysis of Drosophila melanogaster based on ITS 2 rDNA Sequences

Abstract

Abstract The Internal Transcribed Spacer 2 (ITS 2) is a small non-coding region located inside the nuclear ribosomal DNA cluster. ITS 2 sequence variability is widely used in taxonomy and molecular phylogeny. Based on this molecular marker, this study aimed to identify and to construct phylogenetic analysis of D. melanogaster from our laboratory. The phylogenetic tree was constructed after analysis on DNA sequence was conducted by encoding its ITS2 using Unweight Pair Group (UPGMA) method. The result showed that our D. melanogaster wild type and all mutant strains have high similiarity to D. melanogaster 28 S ribosomal RNA gene partial sequence (Gene bank Acc GU 597379.1) (identity score up to 99%). Phylogenetic tree showed that D. melanogaster plum mutant was closely related to D. melanogaster 28 S ribosomal RNA gene partial sequence (Gene bank Acc GU 597379.1), they also belonged to one cluster. Next to this cluster are sepia and clot mutant, in addition to the wild type and black mutant in one cluster. All the mutants and wild type belonged to one clade. While the vestigial mutant stayed in the different clade.