Exploration and selection of salt tolerant phosphate solubilizing bacteria from the Indonesian saline soils

Abstract

Abstract The excessive salt concentration is what prevents saline land from being used. Salinity inhibits plant growth, however this can be remedied by investigating phosphate-solubilizing bacteria (PSB). Non-pathogenic bacteria that can dissolve unavailable P into a form that plants may absorb are known as phosphate solubilizing bacteria. The goal of this study is to find phosphate-solubilizing bacteria that can withstand extremely high salinities. In saline land on Indonesia’s North Coast of Central Java, soil sampling was done. Using pikovskaya selective media, phosphate solubilizing bacteria were isolated using the pour plate method. Eight pure PSB isolates, including BPFRBG2, BPFPT2, BPFDMK1, BPFPKL2, BPFSMG2, BPFBTG2, and BPFKDL2, were obtained as a result of the isolation process. The macro- and microscopic examination of PSB isolates. Following closely behind were the PSB isolates with the strongest tolerance to salt (10.000 ppm NaCl): BPFBTG2, BPFKDL2, BPFSMG2, and BPFPKL2.