Study of The Bacteriological Indicators of Water River Pollution in Kufa and Hilla Cityes

Abstract

Abstract The presence of pathogenic intestinal bacteria in water may have an impact on human health. coli bacteria, and Escherichia coli are commonly used as indicators of water quality. However, the presence of the above symptoms does not always indicate the presence of human enteroviruses. It is critical to understand human enteroviruses in water. Human enteroviruses may withstand changing environmental conditions and persist in during extended periods of time in the environment, becoming the cause of diarrheal diseases. As a result, human pathogenic viruses are increasingly likely to emerge as important warning indicators of water scarcity. Human adenoviruses and other viruses have been recommended as potential indicators for high-quality detection of human-origin organisms that contaminate water systems. The study included measuring the water pollution of the Euphrates River in the cities of Hilla and Kufa by taking water samples in different seasons and locations to determine the extent of pollution, as well as determining the most likely number of fecal coliform bacteria through a series of tests where the highest result was for the total number of aerobic bacteria for the Hilla samples for the site The third was in October, which reached (194C/ml), as well as the Kufa samples in October reached the highest percentage in the third site, which amounted to about (114 C/ml). As for fecal coliform bacteria in Hilla samples, the highest percentage was in October in the third site (450 °C / 100 ml), while in Kufa samples, the highest percentage was in the third site in December (210 °C / 100 ml). Take a sample of water and plant it in a different medium. Bacteria were grown in Petri dishes and cultured on MacConkey agar medium to see the fermented colonies showing that a large proportion of the colonies ferment due to lactose fermentation. Water samples were also grown on Eosin-methylene Blue Agar, a medium used to differentiate between lactose-free fermented bacteria and non-lactose-fermenting bacteria. and is only interested in Gram-negative Lactobacilli that digest lactose: Non-lactose-fermenting bacteria such as Salmonella and Shigella appear as clear, colorless colonies. When E. coli bacteria colonies were detected and medications were tested, a high level of resistance to these treatments was revealed, highlighting the need of cleaning this water since it would have a significant impact on human health.