Fatty acid profiles of Lactobacillus plantarum IIA-1A5 and Lactobacillus acidophilus IIA-2B4

Abstract

Abstract Probiotic lactic acid bacteria have emerged as a natural alternative treatment for cancer in recent years. Indigenous probiotic lactic acid bacteria isolated from beef, Lactobacillus plantarum IIA-1A5 and Lactobacillus acidophilus IIA-2B4, have displayed in vitro anticancer activities against cervical and colorectal cancer cells. Recent studies have suggested that fatty acids such as capric, caprylic, and linoleic acids may play a role in inhibiting tumor development, although the precise mechanisms remain unclear. The aim of this study is to analyze the fatty acid profiles of L. plantarum IIA-1A5 and L. acidophilus IIA-2B4. Intracellular and extracellular fractions of bacterial cultures were obtained using centrifugation. The fatty acid composition was identified using GC-FID. Identified fatty acids were subjected to ANOVA for statistical analysis. Notably, the results show that saturated fatty acid contents exhibited significant differences between all samples, whereas unsaturated fatty acid components varied only between extracellular and intracellular fractions, contributing to the observed diverse anticancer activity. Furthermore, specific saturated fatty acids (C8:0 and C10:0) were found exclusively in L. plantarum IIA-1A5 extracellular or L. acidophilus IIA-2B4 intracellular fraction. These findings might provide valuable insights into the potential therapeutic applications in cancer treatment, warranting further investigation into their underlying mechanisms and clinical potential.