The Estimation of Metabolizable Energy Using an Analysis of Ruminal Fermented Gas Production in Protected Lemuru Fish Oil

Abstract

Abstract This study has purpose to estimate the metabolic energy of protected lemuru fish oil using the rumen fermentation gas production analysis. This study applied a combination of treatments, which is differences in NaOH concentrations and drying methods on the total gas production in vitro. The gas production was determined at the incubation of 0, 2, 4, 8, 12, 24, 48, and 72 hours. This study applied a Completely Randomized Design on 3x2 factorial. The parameters observed included the total gas production, the data were tested using an analysis of variance and continued with the Duncan test. The results of this study indicated that at the incubation time of 48 hours and 72 hours, there was no difference in the interaction between the NaOH concentration and the drying method on the total gas production, and there was a significant difference between the NaOH concentration and the drying method in terms of metabolizable energy (ME). The results of the highest ME value in the protected lemuru fish oil were 10% NaOH concentration and the sun drying method was 4.56 MJ/Kg BK, it can be concluded that the ME estimation is influenced by the yield of incubation gas production for 24 hours.