Genetic diversity analysis of Bitti (Vitex cofasssus), native species Indonesia based on Inter Simple Sequence Repeat (ISSR) marker

Abstract

Abstract Bitti (Vitex cofassus) is a native species in Indonesia and is a superior wood in South Sulawesi. This type is a hardwood that is sought after and exported in large enough quantities. A narrow genetic base requires joint efforts to develop plant varieties, so it is necessary to carry out selection activities to obtain a superior diversity of traits. The most advanced method in assessing genetic diversity is a molecularly based approach using the Inter Simple Sequence Repeat (ISSR) marker. Germplasm exploration was carried out in Pattiroang Village, Kajang District, Bulukumba Regency, South Sulawesi Province. A total of 30 samples were extracted using the Genomic DNA Mini Kit (plant) method with modifications. Ten primers tested obtained six primers that produced bright polymorphic bands, namely UBC 820, UBC 822, UBC 823, UBC 824, UBC 827, and UBC 830. The highest He value is in the UBC 824 primary with a PIC of 0.48, while the lowest He values is in the UBC 827 primer with a PIC value of 0.15. The visualization of the dendrogram shows two large clusters, each consisting of a diverse number of individuals. It indicates a relatively moderate kinship between the types of V. cofassus.