Improvement of mammalian cells performance by addition of glucose for the expression of erythropoietin with 2 additional link in CHO-DG44 cells

Abstract

Abstract The use of recombinant proteins for therapeutic use, currently, have become a standard procedure to fight many diseases and this reality have a great impact on the biotechnology industry. To produce this drug, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. The main goal in recombinant protein production using Chinese hamster ovary (CHO) cells is to achieve both high specific productivity and high cell density. These cells demonstrate a high consumption of glucose and this condition causes rapid depletion this nutrient in the medium. The loss of nutrient such as glucose can result in the limitation of cell growth and viability and finally the loss of quality of the protein. In relation to the previous knowledge, in this work the effect of glucose on cell density and viability of CHO-DG44 cell capable of producing erythropoietin (EPO) was studied. With this in mind 3 mg/liter of glucose was added to the medium at day 4 and 7. The cells were cultured for total 10 days in orbital shaker at the speed of 130 rpm at 37°C and 5% CO2 condition and samples were taken every 2 days. The results showed significantly that the addition of glucose to the medium with the time and concentration mentioned above increased the density and viability of the cell.