Effect of Adding Different Levels of Nano-Zinc Oxide in Tris Diluent on some Traits of Chilled Semen of Bulls from the Artificial Insemination Center in Iraq

Abstract

Abstract This study was conducted in the artificial insemination department for the period from September 2023 until December 2023. It aims to demonstrate effect of adding nano-zinc oxide to Tris diluted on some traits of chilled semen of Holstein bulls. Four bulls from Holstein cows were used in this experiment. Semen was collected from the four bulls for each week at the rate of one ejaculate/bull. The experiment was divided into four treatments: the first treatment, T1, control treatment, diluted Tris without adding an antioxidant, (T2) added 50 μg of Zno/ml diluted, (T3) added 100 μg of ZnO/ml diluted. (T4) 250 μg ZnO/ml diluent was added. It became clear from the results of the current study that the addition of 50 μg of Zno/ml diluted Tris led to a highly significant increase (P>0.01) in the percentage of individual sperm motility after cooling compared to the rest of the treatments. The results showed a highly significant increase (P>0.01) in the percentage of live sperm for the treatment. T2) after cooling compared to the rest of the treatments. The results of the current study showed a highly significant increase (P>0.01) for the T2 treatment when adding 50 μg of Zno/ml diluted in the percentage of plasma membrane integrity compared to the rest of the treatments after cooling. The results of the current study showed a highly significant increase (P>0.01) in acrosome integrity after cooling, as the T2 treatment was excelled compared to other treatments. We conclude from this study that adding nano-zinc oxide (ZnO) at a concentration of (50 μg/ml) to Tris diluent led to an improvement in some traits of semen after cooling for Holstein bulls, namely (individual motility, percentage of live sperm, integrity of the plasma membrane, and integrity of the acrosome.