Expressed sequence tag simple sequence repeats (EST-SSRs) mining and marker development from Leucaena leucocephala root transcriptome

Author:

Faidah A N,Shabrina H

Abstract

Abstract Leucaena leucocephala is a nitrogen-fixing legume and a fast-growing species used for various purposes, including food, medicine, and most recently, energy-source wood. Compared to other commonly planted legumes, this species has a high-stress tolerance, and only one recorded insect infestation. The transcriptome analysis revealed genes involved in the production of stress tolerance compounds in the root. In this study, we attempted to extract simple sequence repeats (SSRs) from the transcriptome data of L. leucocephala root to establish molecular markers. The SSRs mined from L. leucocephala transcriptome deposited in NCBI with accession number GDRZ00000000. We used Krait v.1.3.3 to extract the SSRs and developed the primers. The SSRs cover 0.3% of the total transcriptome sequence, with 21.321 perfect SSRs found and relative density 2908.63 (bp/Mb). The most abundant type of SSRs was found in mononucleotide (39.85%), followed by dinucleotide (33.49%) and trinucleotide (24.75). However, for tetra, penta, and hexanucleotide, the percentage was lower than 2%. The number of primers generated was 8137 primers, with 3969 dinucleotides, 3947 trinucleotides, 150 tetranucleotides, 21 pentanucleotides, and 50 hexanucleotide SSR primers. This finding may help to accelerate stress-tolerant legume improvement in the future.

Publisher

IOP Publishing

Subject

General Engineering

Reference34 articles.

1. Leucaena leucocephala: An underutilized plant for pulp and paper production;Pandey;Genet. Resour. Crop Evol.,2013

2. Phytochemical constituents of the leaves of Leucaena leucocephala from Malaysia;Zayed;Int. J. Pharm. Pharm. Sci.,2016

3. Preliminary study on properties of small diameter wild Leucaena Leucocephala species as potential biomass energy sources;Rasat;ARPN J. Eng. Appl. Sci.,2016

4. Seasonally Distinctive Growth and Drought Stress Functional Traits Enable Leucaena Leucocephala to Successfully Invade a Chinese Tropical Forest;Luo;Trop. Conserv. Sci.,2020

5. Physiological responses of Leucaena leucocephala seedlings to drought stress;Chen;Procedia Eng.,2012

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3