The effect of papain and bromelain enzymes on the growth and feed utilization of post larvae Litopenaeus vannamei

Abstract

Abstract The aim of this research was to evaluate the effect of papain and bromelain enzymes in feed on growth and feed utilization of post larvae Litopenaeus vannamei shrimp. The research was conducted during 23 January to 2 March 2020 at the Fish Hatchery Laboratory, Faculty of Marine and Fisheries, Syiah Kuala University. The completely randomized design consisting of four treatments and three replications were used in this study (A (feed without enzyme); B (feed + 1% papain enzyme); C (feed + 1% bromelain enzyme), and D (feed + 1% papain enzyme + 1% bromelain enzyme)). The shrimp was fed with the tested feed five times on 07.00 AM, 11.00 AM, 03.00 PM, 07.00 PM and 11.00 PM for 42 days. The ANOVA test results showed the addition of papain and bromelain enzymes in the feed had a significant effect on weight gain, daily growth rate and specific growth rate of vannamei shrimp (P<0.05). However, there was no significant effect on its survival, feed conversion and feed efficiency (P>0.05). The best treatment was found in treatment D with the weight gain of 1.15 g, daily growth rate of 0.027 g day-1, specific growth rate of 7.14% day-1, feed conversion ratio of 1.85, and feed efficiency 54.15%, but the highest survival was found at treatment A. It is concluded that the best treatment is a combination between of 1% papain enzyme and 1% bromelain enzyme).