The effectiveness of katuk leaves extract (Sauropus androgynous) as an antibacterial Vibrio sp. on the survival rate and growth of vannamei shrimp larvae (Litopenaeus vannamei)

Abstract

Abstract Vibriosis disease caused by the Vibrio sp. bacteria is one of the problems faced by shrimp farmers, namely the decreased survival rate during the larval period. This study aimed to determine the effect of the ethanol extracts of katuk leaves (Sauropus androgynous) as an antibacterial material on the survival rate and growth performance of vannamei shrimp larvae (Litopenaeus vannamei). This research was conducted at PT. Bibit Unggul Global Gen, Pantai Cermin, Serdang Bedagai North Sumatra, March - April 2019. The extraction evaporation process is carried out at the Unsyiah Faculty of Teacher Training and Education Chemistry Laboratory. Data were analyzed using the Completely Randomized Design (CRD) method with 5 treatment levels and 4 replications. The concentration treatment were is: 0 ppm, 600 ppm, 800 ppm, 1000 ppm and 1200 ppm. The sample used was vannamei shrimp larvae which had been infected with vibrio bacteria, then submerged with katuk leaf extract for 8 minutes. The parameters measured in this study were survival rate (SR%), absolute weight growth (g) and absolute length growth (mm), Total Vibrio Count (TVC Cfu/ml) and water quality. The ANOVA test results obtained that the katuk leaf extract (Sauropus androgynous) had a significant effect on survival (SR) and Total Vibrio Count (TVC) in vannamei shrimp larvae (P<0.05) and had no an significant effect on absolute weight growth and absolute length growth (P>0.05). In this study indicated that concentration 1200 ppm produced the highest the survival rate (93%) and the lowest amount of TVC (533 CFU/ml).