Community structure of Fusarium oxysporum f. sp. cubense in Java and Sumatra based on Cryphonectria parasitica vic primer

Abstract

Abstract Banana production centers in Indonesia are still being invaded by panama disease. Fusarium oxysporum f. sp. cubense (Foc), the causal agents of that disease is reported as genetically varied. Twelve Cryphonectria parasistica vic primer sets were evaluated and used for Foc community structure analyses of Foc population in Java and Sumatra. The results showed that 5 loci (vicia, vic2, vic3a, vic6, and vic7) with 9 (A, B, C, D, E, I, J, K and L) alleles were detected in 38 Foc samples using nine C. parasitica vic primer sets. Two out of nine vic primer sets presumably detected vic loci (vic1a and vic3a). These loci were suspected to relate to the mechanism of apoptosis in Foc somatogamy. Based on loci diversity and allelic interaction systems, 18 haplotypes (1-18) in Foc population from Java and Sumatra were recognized. Haplotype diversity in Sumatra was higher than Java, while alleles relatively spread uniformly. Haplotype-7 and E allele were dominant and were found in Java and Sumatra. Meanwhile, Haplotype-18 and C alleles were specific to Java. Distribution of the dominant haplotype indicated that the island is not a geographic barrier. Three (vic3a, vic6, and vic7) out of nine loci were polymorphic.