Seaweed-Associated Fungi from Sepanjang Beach, GunungKidul, Yogyakarta as Potential Source of Marine Polysaccharides-Degrading Enzymes

Abstract

Abstract Brown algae (Phaeophyceae) and red algae (Rhodophyta) are a group of seaweed that scattered all over the ocean. In addition, previous studies have reported the biotechnological potential of its associated fungi. However, there are only a few studies related to the extracellular enzyme of seaweed-associated fungi. The purposes of this research were to isolated brown algae and red algae associated fungi from Sepanjang Beach, GunungKidul, Yogyakarta, Indonesia, and screen its enzyme production. Padina sp., Asparagopsis sp., and Chondrophycussp. were collected from Sepanjang Beach, GunungKidul, Yogyakarta. Swab tap method on PDA medium and STD medium was conducted to isolate seaweed-associated fungi, while starch agar medium, agar medium, alginate agar medium, and carrageenan agar medium, was utilized to screen the enzyme activity with addition of povidone-iodine 10% reagent. The presence of clear zone around the colony indicated enzyme.