Preliminary Evaluation of Anti Fish Pathogenic Bacteria and Metabolite Profile of Andaliman Fruit (Zanthoxylum acanthopodium DC.) Ethanol Extract

Abstract

Abstract Andaliman (Zanthoxylum acanthopodium) is an endemic plant commonly utilized by people in North Sumatera as an additional food ingredient regarding its intense flavor. The previous study has proven that the andaliman fruit showed antimicrobial, antioxidant, and anti-inflammatory properties. The purposes of this research were to evaluate the antibacterial activity of andaliman fruit ethanol extract against fish pathogenic bacteria and understand its metabolite profile. The extraction was carried out using the maceration method with agitation (115 r.p.m) for 24 hours in ethanol. Thin Layer Chromatography (TLC) and phytochemical test were carried out to characterized the secondary metabolite. The antibacterial activity evaluation was conducted using the paper disc diffusion method against Vibrio anguillarum, Vibrio alginolyticus, and Vibrio harveyi. Ethanol extract of andaliman fruit gave 16 spots on the TLC plate, while the phytochemical results showed the presence of tannin, flavonoid, terpenoid steroid, and quinone. Besides, the evaluation of antibacterial activity gave negative results against fish pathogenic bacteria.