In vitro propagation of Vanda tricolor Lindl. var. suavis protocorm on media containing liquid organic fertilizer as a substitute for MS media

Abstract

Abstract Vanda orchids have a high economic value in the flower industry, so it is necessary to have seeds available at all times. Tissue culture technology offers an important solution to produce plants in large numbers, but it is very costly in media preparation. Hence, it is necessary to have low-cost options for the application of planting media. One way of doing this is to substitute the composition of Murashige and Skoog (MS) media with alternative sources that are more affordable. Liquid organic fertilizer contains macro, micro, and good nutrients for the growth of explants. This study used explants from the protocorm of Vanda tricolor Lindl orchid. var. suavis aged three months planted on media containing liquid organic fertilizer, namely Nasa, Bio88, and Fortune. The parameters observed were the number of shoots and the number of leaves in each treatment. The data were analyzed using the Kruskal-Wallis test at the 5% level, and if there was an effect, it was continued with the Mann-Whitney test. The results showed that liquid organic fertilizer gave different responses to the Vanda tricolor Lindl. var. suavis protocorm. Liquid organic fertilizer has a significant effect on the number of shoots and number of leaves.