Shoot initiation for Macadamia integrifolia explant with tissue culture technique

Abstract

Abstract Macadamia nuts are grown in subtropical and tropical regions and endemic species in Greenland that can be commercially developed in Indonesia. Macadamia’s generative propagation tends to have problems in its seed stock. It often experiences obstacles in field seed stock, and the production requires a long time because it has a thick shell (pericarp). Macadamia initiation needs technology to prevent extinction. One of the propagation is through the technique culture in vitro. This research was conducted to determine the initial response of basic media and to know the response of Macadamia growth in vitro. This research used five media which are Media 1 (DKW with BAP 0.1 ppm, kinetin 0.1ppm), Media 2 (WPM with BAP 1 ppm), media 3 (DKW), media 4 (MS), media 5 (MS with BAP 0.5 ppm). The results showed that media 1 and 3 (DKW media) had a good response for leaf and shoot growth in macadamia explants.