Creation of a device for detecting fluorescence from microfluidic chips for studying biosystems

Abstract

Abstract The article substantiates the need to develop devices using real-time PCR reaction on microfluidic chips for the study of biological systems (objects) to determine environmental pollution and the consequences of these pollution. In situations of radioactive or chemical contamination, it is necessary to detect and eliminate them as soon as possible. For these purposes, PCR is ideally suited, which is currently the leading tool for chemical and biological research. However, most of the currently available PCR devices use tubes or microtiter plates and have some serious disadvantages. Disadvantages: uneven heating/cooling of volumetric systems, analysis speed does not meet the requirements of modern medicine, biology, environmental services, etc., namely, the requirement for rapid analysis. The solution to this problem is microfluidic chips since they are planar systems. Using microfluidic chips, it is possible to analyze more samples in less time. This article presents a layout of a fluorescence detection device using real-time PCR reaction on microfluidic chips. The results of experimental studies on the created layout and their analysis are presented.