Determination of the Efficacy of Broccoli Sprouts Seeds on Growth, Blood Parameters, and Immunological Indicators in Experimentally Immunosuppressed Male Rats

Abstract

Abstract This study was conducted in graduate laboratories, College of Agriculture, University of Baghdad’s and animal house in College of Veterinary Medicine, University of Tikrit to determine the effectiveness of broccoli sprouted seeds on growth parameters, blood components and measurement of immune indicators in male rats with experimentally reduced immunity. The results of the study showed that oral administration of Sandimmun to male albino rats led to a decrease in the weight gain rate of laboratory rats and a decrease in blood components such as the white blood cell count (WBC), red blood cell count (RBC) and hemoglobin concentration level (Hb), while there was a significant increase in the number of platelets (PLT). It also caused a significant decrease in the values of the immunological indicators like IgG, IgA, and IgM. The results showed that feeding on broccoli sprouted seed powder with both concentrations 15% and 30% caused an increase in the weight gain rates to 53.40 and 84.40 g, respectively, compared to the average weights gained for the rats with reduced immunity in treatment G2, which was 15.40 g. The same applies to the effect of feeding on the two treatments G3 and G4. These groups had significant improvement in the blood parameters of rats in both treatments, but the G4 treatment was the best treatment in raising the level of white blood cell counts WBC, which amounted to 14.90x109 cell/ml while the number of RBC erythrocytes was at 8.18x106 cell/ml compared with their numbers in the experimentally immunocompromised group of rats, which were at 15.12x109 cell/ml and 8.06 x106 cells/ml, respectively. While the concentration of hemoglobin (Hb) level was at 13.68 mg/dL, the number of Platelets (PLT) 485x103 platelets / microliter and all these values were significantly similar with their numbers in the values of treatment G1. Also, feeding at a concentrations of 15% and 30% led to increase the immunoglobulins IgG, IgA and IgM in rats, as it caused an increase in their values in treatment G3, which were at 0.48, 0.44, 0.52 mg/dL, respectively. While their values in treatment G4 were at 0.54, 0.47, and 0.56 mg/dL, compared with their values in treatment G2, which amounted to 0.35, 0.33, 0.41 mg/dL. The conclusion of this study is that feeding with broccoli sprouted seed powder improved the growth rates, blood profile parameters and immune globulins in the experimentally reduced immune male rats.