The Effectiveness of the Probiotic Bacillus Spp in Induced Infection with Staphylococcus aureus and the Study of Its Effect on Liver Enzymes, Kidney Function and Microbial Balance of the Gut in Male Rats

Abstract

Abstract The current study aimed to isolate and characterize bacteria of the genus Bacillus. It alsoinvestigate their ability as bio-enhancers, and study their therapeutic effect in vivo in induced infection with Staphylococcusaureus in terms of their impact on the values of liver enzymes Alanine trasaminase, Aspartate aminotranferase, Alkaline phosphatase and kidney functions represented by creatinine and urea, and the microbial balance of the intestine in male rats which were compared with the control groups after a period of 28 days. The study used 20 male Albino Sprague-Dawley rats, ages 8-9 weeks, average weight 148 g. These rats were randomly divided into five groups and four replicates per group. Two types of probiotics of the genus Bacillus were obtained, Bacillus claussi and Bacillus megaterum. The results showed that infection induced by Staphylococcusaureus caused a significant increase at P<0.05 in the values of all liver enzymes ALP, ALT and AST, which were recorded (194.4, 73.17 and 188.6) IU/L compared to the control group, which recorded (180.6, 59.50 and 178.5) IU/L in the same order. The results also showed an increase in creatinine and urea concentrations (52.00 and 0.3000), compared to the control group, which recorded (40.07 and 0.2667) mg/dI. In terms of microbial balance, the results showed an increase in the total number of the tested pathogenic bacteria and a significant decrease in the total number of lactic acid bacteria compared with the control group rats.