Biotherapeutic Effects of Salidroside on Gastric Carcinoma Cells

Abstract

Abstract Salidroside is verified to have tumor inhibitory effects. Nevertheless, the mechanisms are not clarified. The research aims to explore the tumor inhibitory mechanisms of salidroside on gastric carcinoma. The tumor inhibitory effects were examined by the cell viability assay. Flow cytometric analysis was adopted to estimate the cancer cells apoptosis. The expressions of differentiated embryo-chondrocyte expressed gene 1 (DEC1), hypoxia-inducible factor-1α (HIF-1α) and signal transducer and activator of transcription 3 (STAT3) in carcinoma cells were measured by immunohistochemistry assay. Salidroside displayed definite cytotoxicity effects on gastric carcinoma cells in a dose and time dependent manner. Apoptotic percent in carcinoma cells treated with salidroside were significantly increased (P < 0.05). The migrations of cancer cells were suppressed with the increasing doses of salidroside. The expressions of HIF-1α, DEC1 and STAT3 in cancer cells with salidroside treatment were down-regulated. The findings suggested that salidroside suppressed the growth of gastric cancer cells by eliciting the cancer cells apoptosis and lowing the expressions of HIF-1α, DEC1 and STAT3 signal molecules.