Inhibition of Lactobacillus acidophillus, Lactobacillus, Bacteria plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. in the digestive tract in vitro

Abstract

Abstract The bacteria Lactobacillus acidophillus, Lactobacillus plantarum and Bifidobacterium spp. is a lactic acid bacteria that is used as a probiotic. The research was conducted with the aim of knowing the inhibition of the pribiotic bacteria Lactobacillus acidophillus, Lactobacillus plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. Supporting tests such as motility tests, catalase tests, and fermentation tests were also carried out. The media used in this study were: Mueller Hinton Agar (MHA) with the Kirby-Bauer method and Nutrient Broth (NB) media, TSIA, SIM. The control groups used in this study were P1 : Lactobacillus acidophillus, P2 : Lactobacillus plantarum, P3 : Bifidobacterium, P4 : Lactobacillus acidophilus + Bifidobacterium, P5 : Lactobacillus plantarum + Bifidobacterium, P6 : Lactobacillus acidophilus + Lactobacillus plantarum + Bifidobacterium, and P0 as control. The greatest inhibitory power on Escherichia coli bacteria was at P3, P4, P6 at 0.30 mm and Salmonella sp. namely at P4 by 15 mm. While the smallest inhibition on Escherichia coli bacteria is at P5, P7 is 0.2 mm and Salmonella sp. namely at P3, P5, P6 by 0.25 mm.