Improvement of the hormonal and mineral composition of nutrient media used for in vitro regeneration of grape plants

Abstract

Abstract It was established that phytohormones have a positive effect on in vitro regeneration of grape varieties. When using standard nutrient media lacking auxins, cytokinins and gibberellins, shoot regeneration was reduced, and growth and development were inhibited. For in vitro microclonal propagation, MS agarized nutrient media, especially their modifications with 6-BAP (1.0 mg/l) for the first planting, 6-BAP (1.0 mg/l) combined with GK3 (1.0 mg/l) for the transplantation, and IAA (0.5 mg/l) for the second transplantation are optimal.