Effect of partial replacement of Palm Kernel powder, Oyster Mushrooms and commercial enzyme instead of corn in the diets on the blood traits of common carp fingerling fish (Cyprinus carpio L.)

Abstract

Abstract This study was conducted at Al-Anbar University/College of Agriculture/Department of Animal Production / Fish Laboratory for the period from 19/11/2018 to 27/1/2019 to know the effect of adding different levels of Crude palm kernel meal powder and which treated with oyster mushrooms and commercial enzyme mixed as a substitute for corn to the diets of common carp on the hematological parameters blood traits, In the experiment used 20 glass of aquariums basins with dimensions of 70 x 40 x 30 cm with a capacity of 84 liters and it filled with 70 liters were used, in which 140 fish were randomly distributed at a rate of individual weight 2 g ± 19.5 to 10 different treatments and by tow replicate per treatment (7 fish / repeated). The Fish were fed with 4% of their body weight on a pellets diameter of 4.5 mm, on the diets were made in laboratory with a protein content of 27.44% - 30.82%. T2, T3 and T4 coarse dates of raw palm kernel meal 33%, 66%, and 100% respectively were used instead of corn 25%. T5, T6 and T7 dates were treated with Oyster mushrooms with 33, 66, and 100% respectively instead of corn 25%. T8, T9, and T10 dates powder cores were treated with commercial enzymes mixture of 33, 66, and 100% respectively, instead of corn 25%. The results of the study showed, through statistical analysis of the percentage of Packed Cell Volume (PCV) and hemoglobin (Hb) significantly increased (P≤0.05) for T8 (45.50%) compared to T1, T2 and T5, which recorded 38.00, 35.00 and 35.00%, respectively. As for white blood cells, the highest value was in T9, which achieved a rate of 985 ×10³ cells/mm3. For red blood cells, treatment T8, which reached 3,830 106 cells / mm3, recorded a significant increase (P≤0.05) compared to T9 (2.930 106 cells/mm3), no significant differences were recorded with other treatments, and a significant increase (P≤0.01) was observed for T3, recorded at 81.50 ×10³ cells / mm3 In comparison with other treatments, there was also a significant increase (P≤0.01) for the T5 and T6, as it recorded 38.50 and 38.00×10³ cells / mm3, respectively, compared to the T2, T3 and T9, a significant superiority (P≤0.05) was achieved for the Monocyte for T4 and T8 coefficients that recorded 7.5 ×10³ cells/mm3 respectively, compared to T6, T7 and T9, The lowest value, which numbered 4.0 ×10³ cells / mm 3, was recorded for each treatment and no significant differences (P≤0.05) were observed with T1, T2, T3, T5 and T10. As for the level of ALT enzyme in the blood plasma, significant elevation (P≤) was observed. 0.01) for the T5 and T7 treatments, as it recorded 32.00 IU / L, 33.0 IU / L, respectively, compared to the T1, T2, T3, T4, T9, and T10 coefficients. As for the efficacy of the AST enzyme in the blood plasma, it was found that the T7 It was significantly superior (P≤0.01), as it reached 35.00 IU / liter, compared to the rest of the other treatments. We conclude from the study that there is a positive role for adding Crude palm kernel meal powder treated with the commercial enzyme mixed to the diet to improve blood traits and health status of common carp fish.