Optimization of Phenolic Compound Production By Local Aspergillus Niger B1b Isolate

Abstract

Abstract The objective of this study was to optimize the factors for the production of Phenolic Compound from Aspergillus Niger B1b was obtained from laboratories of the College of Agricultural Engineering Sciences, was isolated from soil and genetic diagnosis .Phenolic compounds were produced from locally isolate under different conditions and extract from filtrate of it by ethyl acetate and identified using high performance liquid chromatography instrument (HPLC). Experiments proved that the optimum conditions for phenolic compounds production of Aspergills Niger isolate were, fructose and yeast extract as sources of carbon and nitrogen with concentrations 51 µg/ml.,72 µg/ml respectively. Optimum Temperatures 0, aeration was 200 rpm, inoculum size 710 spore/ml (80, 102.4, 104.3) µg/ml, respectively. while incubation period was 9 days and the pH with concentrations (125, 131.1) µg/ml, respectively.