Effects of lime nitrogen on the fungi community of tobacco soil and its correlation analysis

Abstract

Abstract Soil microorganisms are closely related to soil environmental factors. To explore the effects of lime nitrogen, lime nitrogen combined with biocontrol agents on soil environmental factors and fungi and their correlation in tobacco fields, four treatments were set: 1_1: control group; 1_2: biocontrol agents group; 1_3: lime nitrogen group; 1_4: lime nitrogen with biocontrol agents group. Soil samples were taken during four growth periods of tobacco plants, and fungi were analyzed by high-throughput sequencing. The results showed that both 1_3 and 1_4 group increased the soil pH, OM, available nutrients, effective copper, available zinc, fungi richness and diversity of vigorous and maturity stage, urease, acid phosphatase, invertase activity, flue-cured tobacco economic characteristics, and the relative abundance of beneficial fungi such as Chaetomium, Solicoccozyma of maturity stage. At maturity stage, available zinc, available nitrogen, urease activity was significantly positively correlated with Chaetomium; Invertase activity showed significantly positive correlation with Solicoccozyma. In short, both 1_2 and 1_3 group improved physicochemical properties, more enzyme activity of the soil, which were significantly positively correlation with some beneficial fungi. Therefore, the relative abundance of certain beneficial fungi increased so that the disease resistance of tobacco plant was improved and flue-cured tobacco economic characters were better.