Genetic relationship of kasturi mango (Mangifera casturi Kosterm.) from South Kalimantan and Riau based on microsatellite markers

Abstract

Abstract Mangifera casturi Kosterm is one of the endemic fruits from South Kalimantan. It belongs to the genus Mangifera of the Anacardiaceae family. This study aimed to analyze the genetic relationship of Mangifera casturi from South Kalimantan and Riau using microsatellite markers. Fourteen Mangifera casturi accessions were amplified using fourteen microsatellite loci, and the data produced was utilized to evaluate the genetic relationship. DNA amplification using the multiplex PCR method with fluorescence-labeled dyes on the forward primer will be detected using capillary electrophoresis. Forty-two alleles were obtained from the fourteen loci, averaging three per locus. The observed heterozygosity value (Ho) is 0.24, which is lower than the expected heterozygosity value (He), with an average of 0.48. The fourteen-microsatellite fixation index (FIS) loci ranged between –0.876 and 1. The average Polymorphic information content (PIC) value is 0.39, with the highest found in MC58089 loci (0.65), indicating the SSR marker loci are informative for revealing genetic diversity. The phylogenetic analysis using the neighbor-joining method was constructed into four groups. Kasturi mango from Riau is closely related to Pelipisan and Cuban cultivars from South Kalimantan.