Exploring the influence of maitake (Grifola frondosa) on Mus musculus learning-memory and anxiety behavior

Abstract

Abstract Progressive neurodegeneration affect memory and behavior. One solution to increase neuroprotection is the consumption of medicinal mushrooms such as Grifola frondosa. This study aims to analyze the effect of G. frondosa extract on learning-memory and anxiety levels in mice. Mycelium was cultured in Potato Sucrose Broth medium at room temperature for 14 days. Dried mycelial was extracted using a solvent mixture (22% ethyl acetate and 11% methanol). Learning-memory and anxiety levels were observed using Y-Maze Alternation (Y-Maze) test, Morris Water Maze (MWM) test, Novel Object Recognition test, and Light and Dark Transition test. A total of 18 male mice were divided into three groups and given oral treatment for 14 days: control (distilled water), GF100 (extract of 100 mg/kg BW), and GF200 (crude extract of 200 mg/kg BW). The GF100 and GF200 groups increased spatial learning-memory in Y-Maze and novel object recognition memory, but both treatment groups did not significantly affect anxiety and learning-memory in MWM.