The Primary culture of caudal fin, gill lamella, hepatopancreas and spleen of Osteochilus vittatus

Abstract

Abstract The cell lines play an important role in the development of basic research and applied research. Most of the available cell lines derived from mammalian tissue and only 5% of cell lines derived from fish tissue. This experimental study was conducted to evaluate the potency of caudal fin, gill lamella, hepatopancreas and spleen explant of the Osteochilus vittatus as resources for cell line development. The explants were culture in DMEM/-Ham’s 12 with 15mM HEPES supplemented with 5% L-glutamine, 5% penicillin-streptomycin, and 10% Fetal Bovine Serum for 9 days at 25°C. The results showed that the caudal fin, hepatopancreas, spleen and gill filament cell outgrowth formed confluent monolayer. In addition to monolayer cells, floating life cells were found in the culture media of spleen (1.74×106 cells) and gill lamella (1.54×105 cells). The time needed to reach confluent was significantly different among explant type (p<0.05) with the fastest growth was observed in spleen explant culture. These results indicated that the four tested explants have good potency as resources for cell line development in Osteochilus vittatus.