Determination of T-2 toxin by chemiluminescence enzyme immunoassay

Abstract

Abstract T-2 toxin is a mycotoxin that is harmful to humans and animals. Chemiluminescence enzyme-linked immunosorbent assay (CLEIA) was established for the detection of T-2 toxin. In this study, the antigen coated concentration, antibody dilution proportion, goat anti-mouse IgG-HRP dilution proportion, the concentration of organic solvents, pH value and ionic concentration of the buffer were optimized to obtain the best reaction condition. The experiment have drawn the following conclusions, the 50% inhibitory concentration (IC50) of CLEIA was 0.94ng/ml. The standard was added 1.25ng∼5ng to wheat flour and added 1ng∼5ng to corn flour. The recovery ratios were 83%∼87% and 86%∼107%, the relative standard deviations (RSD) were 3%∼10% and 2 %∼6%, respectively.