Evaluation of the Antibacterial Activity of Aqueous and Alcoholic Extracts of Monks Pepper Seeds on some Pathogenic Bacteria Isolated from Humans

Abstract

Abstract This study has done to evaluate the antimicrobial effects of aqueous and ethanolic extracts of Vitex agnus castus seeds (Lamiaceae family) against several Gram-positive and negative pathogenic bacteria such as Staphylococcus sciuri, Escherichia coli, Enterococcus aerogenes, Citrobacter freundii, Aeromonas sobria and Serratia odorifera were isolated from people suffering from urinary tract infection depended on Laboratory diagnosis procedure via the steps recommended by MaCfadden for diagnosis, from each positive culture take a single colony and then identification according to morphological properties (color, colony shape, texture and edge), using Gram’s stain for detection microscopic properties of bacterial cells also help to differentiate between gram postive and Gram’s negative bacteria. In addition, Confirmation of bacterial study by Vitek 2 compact system. Inhibition zones showed that Enterococcus aerogenes was resistant to all types of aqueous and alcoholic extracts of V.agnus castus seeds and to all concentrations used. Finally, a group of antibiotics was tested against the isolated bacteria under study. The results showed that Erythromycin and Bacitracin were more resistant to the isolated bacteria, in contrast, the antibiotics Azithromycin, Gentamycin, and Ciprofloxacin inhibited the bacteria with varying diameters of inhibition. The current research suggests that the plant includes flavonoids, terpenoids, and steroids that may be useful in the creating phytomedicine to treat the bacterial illnesses that were examined. This scientific article reveald that an watery extract of Vitex agnus castus seeds had great antimicrobial effect against dangerous clinical inflammations.