Evaluation of The Genotype of Barbus Luteus (Heckel, 1843) in Dhi Qar and Basra by ISSR-PCR Technique

Author:

Alomairi Majeed Shnawa Sfyh,Hassooni Hadi Awad,Alkhateeb Ahmed R.

Abstract

Abstract This study was conducted in the Laboratory of Molecular Genetics/Marine Sciences Center/University of Basrah, from 1/1/2019 to 1/1/2020. Tissue samples of Hamri fish were randomly collected from the governorates of Basra (Al-Qurna District) and Dhi Qar (Al-Nasr District). A total samples was 60, including 30 samples from Basra and 30 from Dhi Qar. The results show that the ISSR 12 primer has a genetic morphology 100%, gave the molecular weights in Dhi Qar Governorate (73 and 255) and in Basra Governorate (66, 65). Primer No. (2) also took shape in the province of Dhi Qar (166, 186, 244), and in the province of Basra (194, 237). The primer (ISSR12) gave 8 bundles, with a molecular weight ranging from (73,255) to samples taken from the Dhi Qar and Basra regions. As for the primer (ISSR8).

Publisher

IOP Publishing

Subject

General Engineering

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