Total phenolic and flavonoid contents of Aphanamixis polystachya (Wall.) R.Parker leaf extract and its potential as antioxidant and inhibitor of α-glucosidase

Abstract

Abstract Indonesia is one of the countries with the highest plant diversity in the world, including the Aphanamixis genus, and they use the plants in this genus as traditional medicine. The genus Aphanamixis has a wide variety of compounds with different activities, such as insecticidal, leishmanicidal, antimicrobial, anticancer, cytotoxic against some cancer cells, and antioxidant The focus of this research is to find out about the anti-oxidant, anti-diabetic, and anti-bacterial activities of Aphanamixis polystachya leaf extract from the Bali Botanical Garden plant collection. The antioxidant activity assay was carried out by scavenging the free radical 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenyl ethylamino) propane hydrochloride (DPPH). The antidiabetic assay was carried out by inhibiting the activity of the α-glucosidas eenzyme, while the antibacterial test was carried out by the agar diffusion method. Based on the data generated in this study, the active ingredients of A. polystachya leaf extracts have high potency as antioxidants and α-glucosidase enzyme inhibitors, but the extract was not effective in inhibiting bacterial growth.